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The 14th Scientific Meeting of the Japanese Society of Anti-Aging Medicine

Study of OPH activity in horny layer.

Masamichi Ishigami
Septem-Soken co.
Masayuki Yagi,Riko Mitsuhashi,Yoshikazu Yonei
Doshisha Univ.

< Abstract >

AGEs (advanced glycation end products) which is generated and accumulated by glycation stress acceleration of a living body has an influence on aging and a disease. It learned that the OPH (oxidized protein hydrolase) which decomposition of an oxidized protein and AGEs exists widely in the living body, therefore a possibility which operates on restraint of diabetes and aging is suggested. However, the existence of the OPH in the skin horny layer isn't confirmed. In this study, we focused on a skin horny layer and considered.
A skin horny layer of a healthy 20~60 year old generation was stripped by cellophane tape and added a buffer solution, crude enzyme solution was extracted by ultrasonic. The OPH activity was measured using AAPA (acetyl-L-alanine –p-nitroanillide) and CML (Nε-(carboxymethyl)lysine).
As a result, the OPH activity was found in the skin horny layer. And then, it was considered that the OPH activity rose by the stress of ultraviolet rays.


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