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The 41th Annual Meeting of the Japanese Society for Investigative Dermatology

Roles of intracellular carbonylated protein and oxidized protein hydrolase on reconstruction of basement membrane.

Ryota Mori
Development Laboratory, SEPTEM-SOKEN Co., Ltd.,
Masamichi Ishigami
Development Laboratory, SEPTEM-SOKEN Co., Ltd.,
Masanori Okada
Development Laboratory, SEPTEM-SOKEN Co., Ltd.,
Hitoshi Masaki
Tokyo University of Technology

< Abstract >

Recently, role of carbonylated proteins (CP), which are final products triggering lipid peroxidation, are attracted attention on influence to the process of skin aging, because CP is highly observed in the photoaged skin. Destruction and reconstruction of basement membrane have been examined under the electron microscope, and also observed in photoaged skin. On the other hand, it has been reported that CP are decomposed by oxidized protein hydrolase (OPH) which is a member of serine proteases. However, behavior of OPH for CP accumulation and influence of CP on mRNA expressions of basement membrane member proteins in keratinocytes are still unclear. The aims of this study are to discuss relationship between CP and expressions of basement membrane member proteins and contribution of OPH on CP decomposition in HaCaT keratinocytes. HaCaT keratinocytes exposed to oxidative stress multiply were accumulated CP and down-regulated mRNA expressions of basement membrane member proteins such as type IV and VII collagens HaCaT keratinocytes showed increasing intracellular CP level in the presence of an OPH inhibitor. In conclusion, there is a possibility that oxidative stress or intracellular CP accumulation induces an effect on reconstruction of basement membrane and that OPH plays a role of CP decomposition.

 


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