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Society for Cutaneous Ultrastructure Research

Suppressive effect of Arnica montana on IL-6 production

Akihiro Aioi
SPTM R&D Australia、School of Med, University of Queensland
Roy Kimble, James McMillan
School of Med, University of Queensland

< Abstract >

Interleukin (IL)-6 is a well-known participant in cutaneous inflammatory reactions. Previous cytokine array studies have shown a differential expression of specific cytokines in tissue cultured (myo-)fibroblast populations derived from steady state control dermal tissue (NHF), TGF-b1-treated NHF and hypertrophic scar derived fibroblasts (HSF). Interleukin-6 (IL-6) was one such cytokine identified as upregulated in TGF-b1-treated NHF or HSF fibroblast cultures compared to steady state control dermal fibroblasts. We have examined the suppressive effect of over 200 plant extracts on IL-6, which is a well-known participant in cutaneous inflammatory reactions. IL-6 production from RAW264.7 cells stimulated with lipopolysaccharide (LPS) was suppressed by Arnica extract in a dose-dependent manner, as well as prostaglandin E2 and endothelin-1. According to a recent study (Verma, N. et al., Mol. Cell Biochem. 336(1-2), 127 (2010)), LPS-induced TNF-a production from RAW264.7 a murine macrophage cell line was attenuated, concomitant with inhibition of nuclear translocation of NFkB. Conversely, previous studies demonstrated that TGF-b1 up-regulates collagen synthesis and IL-6 production in dermal fibroblasts via Smad signal transduction. To evaluate the effect of Arnica extract on IL-6 production stimulated by TGF-b1 we used normal human dermal fibroblast (NHDF) and hypertrophic scar fibroblast (HSF) that spontaneously produce TGF-b1. Arnica extract suppressed IL-6 production from both TGF-b1-stimulated NHDF and HSF. Taken together, these results suggest that Arnica extract down-regulates IL-6 production via NFkB and Smad pathways and that Arnica extract is a candidate treatment for skin diseases in which IL-6 plays pivotal roles.

 


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